Chapter 10 Human Oocyte Vitrification

Rienzi L., Cobo A., Ubaldi FM.
Methods Mol Biol. 2017;1568:131-139. doi: 10.1007/978-1-4939-6828-2_10.

Abstract

Discovery and widespread application of successful cryopreservation methods for MII-phase oocytes was one of the greatest successes in human reproduction during the past decade. Although considerable improvements in traditional slow-rate freezing were also achieved, the real breakthrough was the result of introduction of vitrification. Here we describe the method that is most commonly applied for this purpose, provides consistent survival and in vitro developmental rates, results in pregnancy and birth rates comparable to those achievable with fresh oocytes, and does not result in higher incidence of gynecological or postnatal complications.

KEYWORDS:

Donation; Fertility preservation; Human; Oocyte; Technique; Vitrification