SPERM COLLECTION AND PREPARATION

SPERM COLLECTION AND PREPARATION

At the same time as the oocyte collection, the male partner collects the seminal fluid. The collection is carried out in the hospital room.  The seminal fluid is then prepared in the laboratory. The method to be used depends on the characteristics of the seminal fluid to be treated: in the case of normozoospermic samples we proceed with the Swim-up technique; on the other hand in cases of severe oligozoospermia or astenozoospermia it is preferable to adopt the separation on density gradient. The seminal fluid may also contain infectious agents, in which case the technique to be used is preferably the density gradient followed by Swim-up in order to remove the infectious agent as far as possible. However, it must be considered that both procedures cannot guarantee 100% elimination of the potential infectious agent.

In the case of obstructive azoospermia (post-infective, post-surgical or congenital), spermatogenesis is preserved and it is therefore possible to recover spermatozoa surgically. In these circumstances, spermatozoa can be recovered transcutaneously at testicular or epididymis level by a simple intravenous infusion needle or by microsurgical procedure (FNA/PESA-MESA). Conversely, in the case of secretory azoospermia (primary or secondary alteration of spermatogenesis), sperm can be recovered from very small fragments of testicular tissue taken from one or both testicles (TESE). The spermatozoa thus recovered can be processed and used for fertilisation using the ICSI technique.

IVF and ICSI steps

OVARIAN STIMULATION AND ULTRASOUND

AND/OR HORMONAL MONITORING

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OOCYTE

RETRIEVAL

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SPERM COLLECTION

AND PREPARATION

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IN VITRO OOCYTE

INSEMINATION (IVF OR ICSI)

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EMBRYO CULTURE UP

TO THE BLASTOCYST STAGE

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EMBRYO TRANSFER (ET)

AND CRYOPRESERVATION OF EMBRYOS

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