Assessment and management of the risk of SARS-CoV-2 infection in an IVF laboratory Roberta Maggiulli, Adriano Giancani, Gemma Fabozzi, Lisa Dovere, Luisa Tacconi, Maria Giulia Amendola, Danilo Cimadomo, Filippo Maria Ubaldi, Laura Rienzi RBMO – Published: June 27, 2020 DOI:https://doi.org/10.1016/j.rbmo.2020.06.017 ABSTRACT Research question: The study set out to identify corrective measures aimed at reducing the risk of aerosol-mediated viral infection within an IVF laboratory. Design: A failure modes and effect analysis (FMEA) was conducted by a multidisciplinary IVF team. A schematic representation of new protocols and procedures adopted during COVID-19 emergency has been defined, including directives about the behaviour to
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Which key performance indicators are most effective in evaluating and managing an in vitro fertilization laboratory?
Which key performance indicators are most effective in evaluating and managing an in vitro fertilization laboratory? Gemma Fabozzi, Danilo Cimadomo, Roberta Maggiulli, Alberto Vaiarelli, Filippo Maria Ubaldi, and Laura Rienzi Fertility and Sterility 2020 – DOI: https://doi.org/10.1016/j.fertnstert.2020.04.054 ABSTRACT The laboratory is the heart of an in vitro fertilization (IVF) clinic, and a quality management system is critical for its administration. We review the main structural, process, and outcome key performance indicators (KPIs) to provide laboratory managers with concrete tools aimed at enhancing the quality of their work. Three concepts must be stressed when dealing with KPIs in IVF:  always consider
The dawn of the future: 30 years from the first biopsy of a human embryo. The detailed history of an ongoing revolution
The dawn of the future: 30 years from the first biopsy of a human embryo. The detailed history of an ongoing revolution Danilo Cimadomo, Laura Rienzi, Antonio Capalbo, Carmen Rubio, Federica Innocenti, Carmen María García-Pascual, Filippo Maria Ubaldi, and Alan Handyside Human Reproduction Update, pp. 1–21, 2020 – https://doi.org/10.1093/humupd/dmaa019 Submitted on November 30, 2019; resubmitted on March 25, 2020; editorial decision on March 31, 2020; Published: on May 22, 2020 ABSTRACT Following early studies showing no adverse effects, cleavage stage biopsy by zona drilling using acid Tyrode’s solution, and removal of single blastomeres for preimplantation genetic testing (PGT) and identification of
COVID-19 and ART, the view of the Italian Society of Fertility and Sterility and Reproductive Medicine
COVID-19 and ART, the view of the Italian Society of Fertility and Sterility and Reproductive Medicine Alberto Vaiarelli, Carlo Bulletti, Danilo Cimadomo, Andrea Borini, Carlo Alviggi, Silvia Ajossa, Paola Anserini, Gianluca Gennarelli, Maurizio Guido, Paolo E Levi-Setti, Antonio Palagiano, Roberto Palermo, Valeria Savasi, Antonio Pellicer, Laura Rienzi, Filippo M. Ubaldi RBMO Published:April 08, 2020DOI:https://doi.org/10.1016/j.rbmo.2020.04.003 Abstract The COVID-19 pandemic is an unprecedented global situation. As assisted reproductive technology (ART) specialists, we should be cautious, carefully monitoring the situation while contributing by sharing novel evidence to counsel our patients, both pregnant women and would-be mothers. Time to egg collection and drop-out rates
DuoStim – a reproducible strategy to obtain more oocytes and competent embryos in a short time- frame aimed at fertility preservation and IVF purposes. A systematic review
DuoStim – a reproducible strategy to obtain more oocytes and competent embryos in a short time-frame aimed at fertility preservation and IVF purposes. A systematic review Alberto Vaiarelli, Danilo Cimadomo, Cecilia Petriglia, Alessandro Conforti, Carlo Alviggi, Nicolò Ubaldi, Sergio Ledda, Susanna Ferrero, Laura Rienzi and Filippo Maria Ubaldi UPSALA JOURNAL OF MEDICAL SCIENCES Received 08 Nov 2019, Accepted 21 Feb 2020, Published online: 25 Apr 2020 https://doi.org/10.1080/03009734.2020.1734694 ABSTRACT Recent evidence suggests that follicular development occurs in a wave-like model during the ovarian cycle, where up to three cohorts of follicles are recruited to complete folliculogenesis. This understand- ing overtakes
COVID-19: the perspective of Italian embryologists managing the IVF laboratory in pandemic emergency
Human Reproduction – LETTER TO THE EDITOR COVID-19: the perspective of Italian embryologists managing the IVF laboratory in pandemic emergency Lucia De Santis, Attilio Anastasi, Danilo Cimadomo, Francesca Gioia Klinger, Emanuele Licata, Valerio Pisaturo, Laura Sosa Fernandez, and Catello Scarica Human Reproduction, pp. 1–2, 2020 doi:10.1093/humrep/deaa074 Sir, . The COVID-19 pandemic is an unprecedented health emergency, . which confronts us with a radical change in the management of our . work routine and daily life. The epidemic in Italy started on January 30 . and, following the spread of cases throughout the whole country, the . Government declared the
Are there specific patients candidate for oocyte collection during COVID-19 pandemic? Alberto Vaiarelli, Carlo Bulletti, Danilo Cimadomo, Andrea Borini, Cindy Argento, Carlo Alviggi, Silvia Ajossa, Paola Anserini, Gianluca Gennarelli, Maurizio Guido, Paolo Emanuele Levi-Setti, Antonio Palagiano, Roberto Palermo, Valeria Savasi, Antonio Pellicer, Luca Gianaroli, Laura Rienzi, Filippo Maria Ubaldi Fertility and Sterility – April 1°, 2020 At the beginning of January 2020, China announced a new major epidemic foci of coronavirus disease 2019 (COVID-19), now rapidly expanding throughout the world. On March 11 2020, the General Director of the World Health Organization (WHO) declared the COVID-19 disease situation pandemic
Definition of a clinical strategy to enhance the e cacy, e ciency and safety of egg donation cycles with imported vitrified oocytes
Definition of a clinical strategy to enhance the e cacy, e ciency and safety of egg donation cycles with imported vitrified oocytes Laura Rienzi, Danilo Cimadomo, Roberta Maggiulli, Alberto Vaiarelli, Ludovica Dusi, Laura Buffo, Maria Giulia Amendola, Silvia Colamaria, Maddalena Giuliani, Giulietta Bruno, Marta Stoppa, and Filippo Maria Ubaldi Human Reproduction, pp. 1–11, 2020 doi:10.1093/humrep/deaa009; Submitted on October 5, 2019; resubmitted on January 13, 2020; editorial decision on January 17, 2020 ABSTRACT STUDY QUESTION: Which is the most suitable clinical strategy in egg donation IVF cycles conducted with imported donated vitrified oocytes? SUMMARY ANSWER: The importation, and allocation, of at least
Incidence, Origin, and Predictive Model for the Detection and Clinical Management of Segmental Aneuploidies in Human Embryos
Incidence, Origin, and Predictive Model for the Detection and Clinical Management of Segmental Aneuploidies in Human Embryos Laura Girardi, Munevver Serdarogullari, Cristina Patassini, Maurizio Poli, Marco Fabiani, Silvia Caroselli, Onder Coban, Necati Findikli, Fazilet Kubra Boynukalin, Mustafa Bahceci, Rupali Chopra, Rita Canipari, Danilo Cimadomo, Laura Rienzi, Filippo Ubaldi, Eva Hoffmann, Carmen Rubio, Carlos Simon, and Antonio Capalbo The American Journal of Human Genetics online 26 March – 106, 1–10, April 2, 2020 Abstract Despite next-generation sequencing, which now allows for the accurate detection of segmental aneuploidies from in vitro fertilization embryo biopsies, the origin and characteristics of these aneuploidies are
Validation of ART Calculator for Predicting the Number of Metaphase II Oocytes Required for Obtaining at Least One Euploid Blastocyst for Transfer in Couples Undergoing in vitro Fertilization/Intracytoplasmic Sperm Injection
Validation of ART Calculator for Predicting the Number of Metaphase II Oocytes Required for Obtaining at Least One Euploid Blastocyst for Transfer in Couples Undergoing in vitro Fertilization/Intracytoplasmic Sperm Injection Esteves SC, Yarali H, Ubaldi FM, Carvalho JF, Bento FC, Vaiarelli A, Cimadomo D, Özbek İY, Polat M, Bozdag G, Rienzi L, Alviggi C. Front. Endocrinol., 24 January 2020 | https://doi.org/10.3389/fendo.2019.00917 eCollection 2019. PubMed PMID:32038484; PubMed Central PMCID: PMC6992582. ABSTRACT This multicenter study evaluated the reliability of the recently published ART calculator for predicting the minimum number of metaphase II (MII) oocytes (MIImin) to obtain at least one euploid blastocyst in
The effect of ICSI-related procedural timings and operators on the outcome Roberta Maggiulli, Danilo Cimadomo, Gemma Fabozzi, Letizia Papini, Lisa Dovere, Filippo Maria Ubaldi, Laura Rienzi Human Reproduction dez234, https://doi.org/10.1093/humrep/dez234, Received 08 April 2019; Revision received: 11 September 2019; Revision requested: 01 October 2019; Published 09 January 2020 Abstract STUDY QUESTION Do the ICSI-related procedural timings and operators affect the outcomes of an ART cycle? SUMMARY ANSWER The ICSI-related timings and operators do not associate with the mean blastulation rate per cohort of inseminated oocytes and the cumulative delivery rate per concluded cycle, except for a mild association between the times from induction of ovulation to
Luteal phase after conventional stimulation in the same ovarian cycle might improve the management of poor responder patients fulfilling the Bologna criteria: a case series
Luteal phase after conventional stimulation in the same ovarian cycle might improve the management of poor responder patients fulfilling the Bologna criteria: a case series Alberto Vaiarelli, Danilo Cimadomo, Alessandro Conforti, Mauro Schimberni, Maddalena Giuliani, Pietro D’Alessandro, Silvia Colamaria, Carlo Alviggi, Laura Rienzi, and Filippo Maria Ubaldi Fertility and Sterility 2019 Dec 11. pii: S0015-0282(19)32353-2. doi: 10.1016/j.fertnstert.2019.09.012 ABSTRACT OBJECTIVE: To assess the clinical contribution of luteal-phase stimulation (LPS) to follicular-phase stimulation (FPS) in a single ovarian cycle (DuoStim) for poor responder patients fulfilling the Bologna criteria. DESIGN: Observational study (years 2015-2017) including women satisfying ≥2 of the following characteristics: maternal age ≥40 years and/or ≤3 oocytes retrieved after previous conventional stimulation and/or reduced ovarian reserve (i.e., antral follicle count <7 follicles or antimüllerian
Time of morulation and trophectoderm quality are predictors of a live birth after euploid blastocyst transfer: a multicenter study
Time of morulation and trophectoderm quality are predictors of a live birth after euploid blastocyst transfer: a multicenter study Laura Rienzi, Danilo Cimadomo, Arantxa Delgado, Maria Giulia Minasi, Gemma Fabozzi, Raquel del Gallego, Marta Stoppa, Jose Bellver, Adriano Giancani, Marga Esbert, Antonio Capalbo, Jose Remohì, Ermanno Greco, Filippo Maria Ubaldi, and Marcos Meseguer Fertility and Sterility 2019 Dec;112(6):1080-1093.e1. doi: 10.1016/j.fertnstert.2019.07.1322 Abstract Objective: To investigate whether the morphodynamic characterization of a euploid blastocyst’s development allows a higher predic- tion of a live birth after single-embryo-transfer (SET). Design: Observational cohort study conducted in two phases: training and validation. Setting: Private in vitro
The POSEIDON Criteria and Its Measure of Success Through the Eyes of Clinicians and Embryologists Sandro C. Esteves1, Carlo Alviggi, Peter Humaidan, Robert Fischer, Claus Y. Andersen, Alessandro Conforti, Klaus Bühler, Sesh K. Sunkara, Nikolaos P. Polyzos, Daniela Galliano, Michael Grynberg, Hakan Yarali, Irem Y. Özbek, Matheus Roque, Lan N. Vuong, Manish Banker, Laura Rienzi, Alberto Vaiarelli, Danilo Cimadomo and Filippo M. Ubaldi Frontiers in Endocrinology published: 20 November 2019 doi: 10.3389/fendo.2019.00814 Abstract This article represents a viewpoint on the POSEIDON criteria by a group of clinicians and embryologists. Its primary objective is to contextualize the Poseidon criteria and
Similar miRNomic signatures characterize the follicular fluids collected after follicular and luteal phase stimulations in the same ovarian cycle
Similar miRNomic signatures characterize the follicular fluids collected after follicular and luteal phase stimulations in the same ovarian cycle Danilo Cimadomo, Ramona Carmelo, Elvira Immacolata Parrotta, Stefania Scalise, Gianluca Santamaria, Erminia Alviggi, Maria Teresa De Angelis, Gianmarco Sarro, Alberto Vaiarelli, Roberta Venturella, Laura Rienzi, Fulvio Zullo, Filippo Maria Ubaldi, Giovanni Cuda Journal of Assisted Reproduction and Genetics – 2019 Nov 7 – Received: 12 July 2019 / Accepted: 4 October 2019 – https://doi.org/10.1007/s10815-019-01607-6 Abstract Purpose To detect putative differences in the miRNomic profile of follicular fluids collected after follicular-phase-stimulation (FPS-FFs) and paired luteal-phase-stimulation (LPS-FFs) in the same ovarian cycles (DuoStim).
Chromosome errors in human eggs shape natural fertility over reproductive life span Jennifer R. Gruhn, Agata P. Zielinska, Vallari Shukla,, Robert Blanshard, , Antonio Capalbo ,Danilo Cimadomo , Dmitry Nikiforov , Andrew Chi-Ho Chan ,Louise J. Newnham , Ivan Vogel , Catello Scarica , Marta Krapchev , Deborah Taylor , Stine Gry Kristensen , Junping Cheng , Erik Ernst , Anne-Mette Bay Bjørn , Lotte Berdiin Colmorn , Martyn Blayney , Kay Elder , Joanna Liss , Geraldine Hartshorne , Marie Louise Grøndahl , Laura Rienzi , Filippo Ubaldi , Rajiv McCoy , Krzysztof Lukaszuk , Claus Yding Andersen ,
Contribution of cryopreservation to the cumulative live birth rate: a large multicentric cycle-based data analysis from the Italian National Registry
Contribution of cryopreservation to the cumulative live birth rate: a large multicentric cycle-based data analysis from the Italian National Registry Scaravelli G, Levi-Setti P.E, Livi C., La Sala G., Ubaldi F.M, Greco E., Coccia M.E, Borini A., Revelli A., Ricci G., Vigiliano V., De Luca R., Bolli S., Rienzi L., Spoletini R., Drovanti A., Benini F., Villani M.T, Albricci L., Varricchio M.T, Basile V., Cattoli M., Nizzi A., Skerk K., Speziale L., Fiaccavento S.. Journal of Assisted Reproduction and Genetics, 2019 Aug 28. doi: 10.1007/s10815-019-01566-y ABSTRACT PURPOSE: To estimate the contribution of cryopreservation to the cumulative live birth rate (CLBR)
Definition and validation of a custom protocol to detect miRNAs in the spent media after blastocyst culture: searching for biomarkers of implantation
Definition and validation of a custom protocol to detect miRNAs in the spent media after blastocyst culture: searching for biomarkers of implantation Danilo Cimadomo, Laura Rienzi, Adriano Giancani, Erminia Alviggi, Ludovica Dusi, Rita Canipari, Laila Noli, Dusko Ilic, Yacoub Khalaf, Filippo Maria Ubaldi, and Antonio Capalbo Human Reproduction, 2019 Aug 16 – pp. 1–16, 2019 doi:10.1093/humrep/dez119 ABSTRACT STUDY QUESTION: Can miRNAs be reliably detected in the spent blastocyst media (SBM) after IVF as putative biomarkers of the implantation potential of euploid embryos? SUMMARY ANSWER: Adjustment of the data for blastocyst quality and the day of full-expansion hinders the predictive
Management of Women With an Unexpected Low Ovarian Response to Gonadotropin Conforti A., Esteves S.C, Cimadomo D., Vaiarelli A., Di Rella F., Ubaldi F.M, Zullo F., De Placido G., Alviggi C. Front Endocrinol (Lausanne). 2019 Jun 27;10:387. doi: 10.3389/fendo.2019.00387. eCollection 2019. Abstract POSEIDON groups 1 and 2 patients respond poorly (<4 oocytes retrieved) or sub-optimally (4-9 oocytes retrieved) to gonadotropin stimulation despite the presence of adequate ovarian parameters, which negatively affect their cumulative chances of delivering a baby using Assisted Reproductive Technology. A polygenic trait involving gonadotropins and/or their receptors seems to be the primary pathophysiology mechanism explaining this phenomenon.
Human Blastocyst Biopsy and Vitrification Maggiulli R., Giancani A., Cimadomo D., Ubaldi F.M, Rienzi L. Journal of Visualized Experiments 2019 Jul 26;(149). doi: 10.3791/59625. Abstract Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the diagnostic techniques and to transfer the selected embryo(s) on a physiological endometrium in a following natural cycle. A biopsy approach encompassing the sequential opening of the zona pellucida and retrieval of 5-10 trophectoderm cells (ideally 7-8) limits both the number of manipulations required
Looking past the appearance: a comprehensive description of the clinical contribution of poor-quality blastocysts to increase live birth rates during cycles with aneuploidy testing
Looking past the appearance: a comprehensive description of the clinical contribution of poor-quality blastocysts to increase live birth rates during cycles with aneuploidy testing Danilo Cimadomo, Daria Soscia, Alberto Vaiarelli, Roberta Maggiulli, Antonio Capalbo, Filippo Maria Ubaldi, and Laura Rienzi Human Reproduction, Volume 34, Issue 7, 2019 July 8, Pages 1206–1214, https://doi.org/10.1093/humrep/dez078 Abstract STUDY QUESTION: Which are the clinical benefits and risks of including poor-quality blastocysts (PQBs) in the cohort of biopsied embryos during a cycle with preimplantation genetic testing for aneuploidies (PGT-A)? SUMMARY ANSWER: PQBs show a worse prognosis with respect to sibling non-PQBs, but their clinical use allows
Sperm DNA fragmentation to predict embryo development, implantation and miscarriage: still an open question
Sperm DNA fragmentation to predict embryo development, implantation and miscarriage: still an open question Rienzi L., Mazzilli R., Ubaldi F.M. Fertil. Steril. 2019 Jul 1. pii: S0015-0282(19)30472-8. doi: 10.1016/j.fertnstert.2019.05.016. Abstract Male factor infertility is responsible for approximately half of all causes of infertility. In the last decade, more attention has been given to sperm DNA fragmentation (SDF) as a potential cause of unexplained infertility. The increased DNA damage in spermatozoa from men with oligozoospermia seems to be associated with both a reduced chance of natural conception and an increased risk of early pregnancy loss (1). Nevertheless, the role of SDF during in vitro fertilization
Pharmacogenetics of FSH Action in the Female Conforti A., Vaiarelli A., Cimadomo D., Bagnulo F., Peluso S., Carbone L., Di Rella F., De Placido G., Ubaldi F.M., Huhtaniemi I., Alviggi C. Front. Endocrinol., 26 June 2019 | https://doi.org/10.3389/fendo.2019.00398 Abstract The purpose of a pharmacogenomic approach is to tailor treatment on the basis of an individual human genotype. This strategy is becoming increasingly common in medicine, and important results have been obtained in oncologic and antimicrobial therapies. The rapid technological developments and availability of innovative methodologies have revealed the existence of numerous genotypes that can influence the action of medications and give rise to the idea that a true “individualized” approach could
Embryonic cell-free DNA versus trophectoderm biopsy for aneuploidy testing: concordance rate and clinical implications
Embryonic cell-free DNA versus trophectoderm biopsy for aneuploidy testing: concordance rate and clinical implications Rubio C., Rienzi L., Navarro-Sánchez L., Cimadomo D., García-Pascual CM., Albricci L., Soscia D., Valbuena D., Capalbo A., Ubaldi F.M., Simón C. Fertil Steril. 2019 Sep;112(3):510-519. doi: 10.1016/j.fertnstert.2019.04.038. Epub 2019 Jun 11 ABSTRACT OBJECTIVE: To study whether embryonic cell-free DNA (cfDNA) in spent blastocyst media is representative of the chromosomal constitution of a blastocyst. DESIGN: Pilot prospective blinded study. SETTING: In vitro fertilization center and genetics laboratory. PATIENT(S): A total of 115 trophectoderm (TE) biopsies and spent blastocyst media (SBM) from 46 patients with ages ranging from 32 to 46 years, whose indications for preimplantation genetic testing of aneuploidy
Non-invasive mitochondrial DNA quantification on day 3 predicts blastocyst development: a prospective, blinded, multi-centric study
Non-invasive mitochondrial DNA quantification on day 3 predicts blastocyst development: a prospective, blinded, multi-centric study Stigliani S, Orlando G, Massarotti C, Casciano I, Bovis F, Anserini P, Ubaldi FM, Remorgida V, Rienzi L, Scaruffi P Mol Hum Reprod. 2019 Jun 7. pii: gaz032. doi: 10.1093/molehr/gaz032. ABSTRACT In ART, embryo quality evaluation is routinely based on morphological criteria. We previously demonstrated that the mitochondrial DNA (mtDNA)/genomic DNA (gDNA) ratio in culture medium was significantly associated with embryo quality and viability potential. The purpose of this prospective, blinded, multi-centric study was to validate the use of mtDNA/gDNA ratio in day 3 spent medium as a predictor of human embryo developmental competence.