Publications

PUBLICATIONS

The scientific quality of our group has led to the publication of over 200 articles in international scientific journals.

"You care better where you research".

Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance

Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance Rienzi L, Gracia C, Maggiulli R, LaBarbera AR, Kaser DJ, Ubaldi FM, Vanderpoel S, Racowsky C Human Reproduction Update – Received June 2, 2016. Revision received September 15, 2016. Accepted October 14, 2016. First published online: November 8, 2016 Abstract BACKGROUND Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a

Human embryos created by embryo splitting secrete significantly lower levels of miRNA-30c

Human embryos created by embryo splitting secrete significantly lower levels of miRNA-30c Laila Noli, Antonio Capalbo, Yaser Dajani, Danilo Cimadomo, Jean Bvumbe, Laura Rienzi, Filippo Maria Ubaldi, Caroline Ogilvie, Yacoub Khalaf, and Dusko Ilic STEM CELLS AND DEVELOPMENT Volume 25, Number 24, 2016 Epub 2016 Oct 17. Mary Ann Liebert, Inc. DOI: 10.1089/scd.2016.0212 Abstract Studies reporting term pregnancy and the production of genetically identical offspring from isolated blastomeres of early stage embryos have been carried out in small and large animals. However, very little is known about the effects of embryo splitting on the development and reproductive competency of human

Detecting mosaicism in trophectoderm biopsies: current challenges and future possibilities

Detecting mosaicism in trophectoderm biopsies: current challenges and future possibilities Antonio Capalbo, Filippo Maria Ubaldi, Laura Rienzi, Richard Scott, Nathan Treff Hum. Reprod. (2016) doi: 10.1093/humrep/dew250 – Received April 16, 2016, Revision received September 8, 2016, Accepted September 12, 2016, First published online: October 13, 2016 Abstract Embryonic mosaicism, defined as the presence of karyotypically distinct cell lines within an embryo, has been frequently reported with a high incidence in preimplantation embryos derived from IVF and is thought to be one of the major biological limitations for the routine application of PGD for aneuploidies (PGD-A). The incidence of mosaicism in preimplantation embryos is in fact

Pre-implantation genetic testing in ART: who will benefit and what is the evidence?

Pre-implantation genetic testing in ART: who will benefit and what is the evidence? Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, and Filippo Maria Ubaldi Journal of Assisted Reproduction and Genetics 2016 October; 33 (10):1273-1278 Abstract Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles Danilo Cimadomo, Filippo Maria Ubaldia, Antonio Capalboa, Roberta Maggiulli, Catello Scarica, Stefania Romano, Cristina Poggiana, Daniela Zuccarello, Adriano Giancani, Alberto Vaiarelli, Laura Rienzi  Reproductive BioMedicine Online Volume 33, Issue 3, September 2016, Pages 360–369 Abstract Preimplantation genetic diagnosis and aneuploidy testing (PGD/PGS) use is constantly growing in IVF, and embryo/biopsy traceability during the additional laboratory procedures needed is pivotal. An electronic witnessing system (EWS), which showed a significant value in decreasing mismatch occurrence and increasing detection possibilities during standard care IVF, still does not guarantee the same level of

45,X product of conception after preimplantation genetic diagnosis and euploid embryo transfer: evidence of a spontaneous conception confirmed by DNA fingerprinting

45,X product of conception after preimplantation genetic diagnosis and euploid embryo transfer: evidence of a spontaneous conception confirmed by DNA fingerprinting Daniela Bettio, Antonio Capalbo, Elena Albani, Laura Rienzi, Valentina Achille, Anna Venci, Filippo Maria Ubaldi, and Paolo Emanuele Levi Setti Reproductive Biology Endocrinology. 2016; 14 (1): 55. Published online 2016 Sep 6 Abstract Background Preimplantation genetic screening (PGS) provides an opportunity to eliminate a potential implantation failure due to aneuploidy in infertile couples. Some studies clearly show that twins following single embryo transfer (SET) can be the result of a concurrent natural conception and an incidence as high as 1 in 5 twins has been

Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin

Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin Stem Cells and Development, published in Volume: 25 Issue 18: September 1, 2016, pages 1366-1375 Petrova A., Capalbo A., Jacquet L., Hazelwood-Smith S., Dafou D., Hobbs C., Arno M., Farcomeni A., Devito L., Badraiq H., Simpson M., McGrath JA., Di WL., Cheng JB., Mauro TM., Ilic D. Abstract The epigenetic background of pluripotent stem cells can influence transcriptional and functional behavior. Most of these data have been obtained in standard monolayer cell culture systems. In this study, we used exome sequencing, array comparative genomic hybridization (CGH), miRNA array, DNA methylation array, three-dimensional (3D) tissue engineering, and immunostaining to conduct a comparative

The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists

The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, Grifo J, Handyside A, Harper J, Kokkali G, Mastenbroek S, Meldrum D, Meseguer M, Montag M, Munné S, Rienzi L, Rubio C, Scott K, Scott R, Simon C, Swain J, Treff N, Ubaldi F, Vassena R, Vermeesch JR, Verpoest W, Wells D, Geraedts J. MHR: Basic science of reproductive medicine, 2016 Aug.; Volume 22, Issue 8

Pre-implantation genetic testing in ART: who will benefit and what is the evidence?

Pre-implantation genetic testing in ART: who will benefit and what is the evidence? Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, Filippo Maria Ubaldi Journal of Assisted Reproduction and Genetics – October 2016, Volume 33, Issue 10, pp 1273–1278 – First Online: 05 August 2016 Abstract Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first version based on

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

5Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials Ioannis A. Sfontouris, Wellington P. Martins, Carolina O Nastri, Iara G. R. Viana, Paula A. Navarro, Nick Raine-Fenning, Sheryl van der Poel, Laura Rienzi, Catherine Racowsky Journal of Assisted Reproduction and Genetics, October 2016, Volume 33, Issue 10, p. 1261-1272 – First Online: 5 August 2016 ABSTRACT Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management  Antonio Capalbo, Valeria Romanelli, Danilo Cimadomo, Laura Girardi, Marta Stoppa, Lisa Dovere, Domenico Dell’Edera, Filippo Maria Ubaldi, Laura Rienzi Journal of Assisted Reproduction and Genetics October 2016, Volume 33, Issue 10, pp 1279–1286 – First Online: 16 July 2016 Abstract For an IVF clinic that wishes to implement preimplantation genetic diagnosis for monogenic diseases (PGD) and for aneuploidy testing (PGD-A), a global improvement is required through all the steps of an IVF treatment and patient care. At present, CCS (Comprehensive Chromosome Screening)-based trophectoderm (TE) biopsy has been demonstrated as a safe, accurate and reproducible approach to conduct PGD-A and possibly also PGD

Electronic witness system in IVF - patients perspective

Electronic witness system in IVF – patients perspective Marina Forte, Federica Faustini, Roberta Maggiulli, Catello Scarica, Stefania Romano, Christian Ottolini, Alessio Farcomeni, Antonio Palagiano, Antonio Capalbo, Filippo Maria Ubaldi, Laura Rienzi Journal of Assisted Reproduction and Genetics, September 2016, Volume 33, Issue 9, pp 1215–1222 – First Online: 07 July 2016 Abstract  Objective The objective of this study is to evaluate patient concerns about in vitro fertilization (IVF) errors and electronic witness systems (EWS) satisfaction. Design The design of this study is a prospective single-center cohort study. Setting The setting of this study was located in the private IVF center. Patient(s) Four hundred eight infertile patients attending an IVF cycle at a GENERA center in Italy were

Generation of meiomaps of genome - wide recombination and chromosome segregation in human oocytes

Generation of meiomaps of genome – wide recombination and chromosome segregation in human oocytes Christian S Ottolini, Antonio Capalbo, Louise Newnham, Danilo Cimadomo, Senthilkumar A. Natesan, Eva R. Hoffmann, Filippo M. Ubaldi, Laura Rienzi and Alan H. Handyside  Nature Protocols 11, pages 1229–1243 (2016) doi:10.1038/nprot.2016.075 – Published online16 June 2016 Abstract We have developed a protocol for the generation of genome-wide maps (meiomaps) of recombination and chromosome segregation for the three products of human female meiosis: the first and second polar bodies (PB1 and PB2) and the corresponding oocyte. PB1 is biopsied and the oocyte is artificially activated by exposure

Follicular versus luteal phase ovarian stimulation during the same menstrual cycle (DuoStim) in a reduced ovarian reserve population results in a similar euploid blastocyst formation rate: new insight in ovarian reserve exploitation

Follicular versus luteal phase ovarian stimulation during the same menstrual cycle (DuoStim) in a reduced ovarian reserve population results in a similar euploid blastocyst formation rate: new insight in ovarian reserve exploitation   Filippo Maria Ubaldi, Antonio Capalbo, Alberto Vaiarelli, Danilo Cimadomo, Silvia Colamaria, Carlo Alviggi, Elisabetta Trabucco, Roberta Venturella, Gabor Vajta, Laura Rienzi Fertility and Sterility, Volume 105 Issue 6, June 2016, pages 1488-1495.e1 Objective To compare the euploid blastocyst formation rates obtained after follicular phase (FP) versus luteal phase (LP) stimulation performed in the same menstrual cycle in a preimplantation genetic diagnosis for aneuploidy testing (PGD-A) program in patients

Revised guidelines for good practice in IVF laboratories (2015)

Revised guidelines for good practice in IVF laboratories (2015) The ESHRE Guideline Group on Good Practice in IVF Labs, De los Santos MJ, Apter S, Coticchio G, Debrock S, Lundin K, Plancha CE, Prados F, Rienzi L, Verheyen G, Woodward B, Vermeulen N. Human Reproduction, Volume 31, Issue 4, Pp. 685-686 – Received December 18, 2015. Revision received December 18, 2015. Accepted January 11, 2016.  Epub 2016 Feb 17. ABSTRACT STUDY QUESTION: Which recommendations can be provided by the European Society of Human Reproduction and Embryology Special Interest Group (ESHRE SIG) Embryology to support laboratory specialists in the organization and management of IVF laboratories and the optimization of IVF patient care? SUMMARY ANSWER: Structured in 13 sections, the guideline development group formulated recommendations for good practice in

New approaches for multifactor preimplantation genetic diagnosis of monogenic diseases and aneuploidies from a single biopsy

New approaches for multifactor preimplantation genetic diagnosis of monogenic diseases and aneuploidies from a single biopsy Capalbo A., Rienzi L., Ubaldi F.M. GENERA, Centers for Reproductive Medicine, Rome, Italy GENETYX, Molecular Genetics Laboratory, Marostica, Italy   Fertility and Sterility 2016 Feb. – Volume 105, Issue 2, Pages 297–298 Abstract Preimplantation genetic diagnosis (PGD) is an alternative to spontaneous conception and chorionic villus sampling or amniocentesis for couples at risk of transmitting a defined genetic disorder. It involves the diagnosis of genetic disease on embryo biopsies during an IVF cycle before a clinical pregnancy has been established and represents a preferred reproductive option

The Impact of Biopsy on Human Embryo Developmental Potential during Preimplantation Genetic Diagnosis

33The Impact of Biopsy on Human Embryo Developmental Potential during Preimplantation Genetic Diagnosis Danilo Cimadomo, Antonio Capalbo, Filippo Maria Ubaldi, Catello Scarica, Antonio Palagiano, Rita Canipari, and Laura Rienzi BioMed Research International, 2016 – Published online 2016 January 28 Abstract Preimplantation Genetic Diagnosis and Screening (PGD/PGS) for monogenic diseases and/or numerical/structural chromosomal abnormalities is a tool for embryo testing aimed at identifying nonaffected and/or euploid embryos in a cohort produced during an IVF cycle. A critical aspect of this technology is the potential detrimental effect that the biopsy itself can have upon the embryo. Different embryo biopsy strategies have been proposed.

Early protein profile of human embryonic secretome

1Early protein profile of human embryonic secretome Foresta C, Ubaldi FM, Rienzi L, Franchin C, Pivato M, Romano S, Guidolin D, De Caro R, Ferlin A, De Toni L. Front Biosci (Landmark Ed). 2016 Jan 1;21:620-34 Abstract Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the 3rd day of in vitro culture. 70 proteins were identified in the medium of 48 embryos. Validation by protein array on two pools

Artificial oocyte activation with calcium ionophore does not cause a widespread increase in chromosome segregation errors in the second meiotic division of the oocyte

Artificial oocyte activation with calcium ionophore does not cause a widespread increase in chromosome segregation errors in the second meiotic division of the oocyte  Antonio Capalbo, Christian S. Ottolini, Darren K. Griffin, Filippo Maria Ubaldi, Alan H. Handyside, Laura Rienzi Fertil Steril. 2016 Mar; 105(3):807-814.e2. doi: 10.1016/j.fertnstert.2015.11.017. Epub 2015 Dec 1   ABSTRACT Objective To study the effect of artificial oocyte activation (AOA) on chromosome segregation errors in the meiotic divisions. Design Prospective cohort study with historical control. Setting Private/academic IVF centers. Patient(s) Fifty-six metaphase II oocytes were donated from 12 patients who had undergone IVF between June 2008 and May 2009.