Publications

PUBLICATIONS

The scientific quality of our group has led to the publication of over 200 articles in international scientific journals.

“You care better where you research”.

Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance

Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance Rienzi L, Gracia C, Maggiulli R, LaBarbera AR, Kaser DJ, Ubaldi FM, Vanderpoel S, Racowsky C Human Reproduction Update – Received June 2, 2016. Revision received September 15, 2016. Accepted October 14, 2016. First published online: November 8, 2016 Abstract BACKGROUND Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a

Human embryos created by embryo splitting secrete significantly lower levels of miRNA-30c

Human embryos created by embryo splitting secrete significantly lower levels of miRNA-30c Laila Noli, Antonio Capalbo, Yaser Dajani, Danilo Cimadomo, Jean Bvumbe, Laura Rienzi, Filippo Maria Ubaldi, Caroline Ogilvie, Yacoub Khalaf, and Dusko Ilic STEM CELLS AND DEVELOPMENT Volume 25, Number 24, 2016 Epub 2016 Oct 17. Mary Ann Liebert, Inc. DOI: 10.1089/scd.2016.0212 Abstract Studies reporting term pregnancy and the production of genetically identical offspring from isolated blastomeres of early stage embryos have been carried out in small and large animals. However, very little is known about the effects of embryo splitting on the development and reproductive competency of human

Detecting mosaicism in trophectoderm biopsies: current challenges and future possibilities

Detecting mosaicism in trophectoderm biopsies: current challenges and future possibilities Antonio Capalbo, Filippo Maria Ubaldi, Laura Rienzi, Richard Scott, Nathan Treff Hum. Reprod. (2016) doi: 10.1093/humrep/dew250 – Received April 16, 2016, Revision received September 8, 2016, Accepted September 12, 2016, First published online: October 13, 2016 Abstract Embryonic mosaicism, defined as the presence of karyotypically distinct cell lines within an embryo, has been frequently reported with a high incidence in preimplantation embryos derived from IVF and is thought to be one of the major biological limitations for the routine application of PGD for aneuploidies (PGD-A). The incidence of mosaicism in preimplantation embryos is in fact

Pre-implantation genetic testing in ART: who will benefit and what is the evidence?

Pre-implantation genetic testing in ART: who will benefit and what is the evidence? Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, and Filippo Maria Ubaldi Journal of Assisted Reproduction and Genetics 2016 October; 33 (10):1273-1278 Abstract Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles Danilo Cimadomo, Filippo Maria Ubaldia, Antonio Capalboa, Roberta Maggiulli, Catello Scarica, Stefania Romano, Cristina Poggiana, Daniela Zuccarello, Adriano Giancani, Alberto Vaiarelli, Laura Rienzi  Reproductive BioMedicine Online Volume 33, Issue 3, September 2016, Pages 360–369 Abstract Preimplantation genetic diagnosis and aneuploidy testing (PGD/PGS) use is constantly growing in IVF, and embryo/biopsy traceability during the additional laboratory procedures needed is pivotal. An electronic witnessing system (EWS), which showed a significant value in decreasing mismatch occurrence and increasing detection possibilities during standard care IVF, still does not guarantee the same level of

45,X product of conception after preimplantation genetic diagnosis and euploid embryo transfer: evidence of a spontaneous conception confirmed by DNA fingerprinting

45,X product of conception after preimplantation genetic diagnosis and euploid embryo transfer: evidence of a spontaneous conception confirmed by DNA fingerprinting Daniela Bettio, Antonio Capalbo, Elena Albani, Laura Rienzi, Valentina Achille, Anna Venci, Filippo Maria Ubaldi, and Paolo Emanuele Levi Setti Reproductive Biology Endocrinology. 2016; 14 (1): 55. Published online 2016 Sep 6 Abstract Background Preimplantation genetic screening (PGS) provides an opportunity to eliminate a potential implantation failure due to aneuploidy in infertile couples. Some studies clearly show that twins following single embryo transfer (SET) can be the result of a concurrent natural conception and an incidence as high as 1 in 5 twins has been

Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin

Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin Stem Cells and Development, published in Volume: 25 Issue 18: September 1, 2016, pages 1366-1375 Petrova A., Capalbo A., Jacquet L., Hazelwood-Smith S., Dafou D., Hobbs C., Arno M., Farcomeni A., Devito L., Badraiq H., Simpson M., McGrath JA., Di WL., Cheng JB., Mauro TM., Ilic D. Abstract The epigenetic background of pluripotent stem cells can influence transcriptional and functional behavior. Most of these data have been obtained in standard monolayer cell culture systems. In this study, we used exome sequencing, array comparative genomic hybridization (CGH), miRNA array, DNA methylation array, three-dimensional (3D) tissue engineering, and immunostaining to conduct a comparative

The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists

The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, Grifo J, Handyside A, Harper J, Kokkali G, Mastenbroek S, Meldrum D, Meseguer M, Montag M, Munné S, Rienzi L, Rubio C, Scott K, Scott R, Simon C, Swain J, Treff N, Ubaldi F, Vassena R, Vermeesch JR, Verpoest W, Wells D, Geraedts J. MHR: Basic science of reproductive medicine, 2016 Aug.; Volume 22, Issue 8

Pre-implantation genetic testing in ART: who will benefit and what is the evidence?

Pre-implantation genetic testing in ART: who will benefit and what is the evidence? Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, Filippo Maria Ubaldi Journal of Assisted Reproduction and Genetics – October 2016, Volume 33, Issue 10, pp 1273–1278 – First Online: 05 August 2016 Abstract Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first version based on

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

5Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials Ioannis A. Sfontouris, Wellington P. Martins, Carolina O Nastri, Iara G. R. Viana, Paula A. Navarro, Nick Raine-Fenning, Sheryl van der Poel, Laura Rienzi, Catherine Racowsky Journal of Assisted Reproduction and Genetics, October 2016, Volume 33, Issue 10, p. 1261-1272 – First Online: 5 August 2016 ABSTRACT Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International