Publications

PUBLICATIONS

The scientific quality of our group has led to the publication of over 200 articles in international scientific journals.

“You care better where you research”.

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management  Antonio Capalbo, Valeria Romanelli, Danilo Cimadomo, Laura Girardi, Marta Stoppa, Lisa Dovere, Domenico Dell’Edera, Filippo Maria Ubaldi, Laura Rienzi Journal of Assisted Reproduction and Genetics October 2016, Volume 33, Issue 10, pp 1279–1286 – First Online: 16 July 2016 Abstract For an IVF clinic that wishes to implement preimplantation genetic diagnosis for monogenic diseases (PGD) and for aneuploidy testing (PGD-A), a global improvement is required through all the steps of an IVF treatment and patient care. At present, CCS (Comprehensive Chromosome Screening)-based trophectoderm (TE) biopsy has been demonstrated as a safe, accurate and reproducible approach to conduct PGD-A and possibly also PGD

Electronic witness system in IVF - patients perspective

Electronic witness system in IVF – patients perspective Marina Forte, Federica Faustini, Roberta Maggiulli, Catello Scarica, Stefania Romano, Christian Ottolini, Alessio Farcomeni, Antonio Palagiano, Antonio Capalbo, Filippo Maria Ubaldi, Laura Rienzi Journal of Assisted Reproduction and Genetics, September 2016, Volume 33, Issue 9, pp 1215–1222 – First Online: 07 July 2016 Abstract  Objective The objective of this study is to evaluate patient concerns about in vitro fertilization (IVF) errors and electronic witness systems (EWS) satisfaction. Design The design of this study is a prospective single-center cohort study. Setting The setting of this study was located in the private IVF center. Patient(s) Four hundred eight infertile patients attending an IVF cycle at a GENERA center in Italy were

Generation of meiomaps of genome - wide recombination and chromosome segregation in human oocytes

Generation of meiomaps of genome – wide recombination and chromosome segregation in human oocytes Christian S Ottolini, Antonio Capalbo, Louise Newnham, Danilo Cimadomo, Senthilkumar A. Natesan, Eva R. Hoffmann, Filippo M. Ubaldi, Laura Rienzi and Alan H. Handyside  Nature Protocols 11, pages 1229–1243 (2016) doi:10.1038/nprot.2016.075 – Published online16 June 2016 Abstract We have developed a protocol for the generation of genome-wide maps (meiomaps) of recombination and chromosome segregation for the three products of human female meiosis: the first and second polar bodies (PB1 and PB2) and the corresponding oocyte. PB1 is biopsied and the oocyte is artificially activated by exposure

Follicular versus luteal phase ovarian stimulation during the same menstrual cycle (DuoStim) in a reduced ovarian reserve population results in a similar euploid blastocyst formation rate: new insight in ovarian reserve exploitation

Follicular versus luteal phase ovarian stimulation during the same menstrual cycle (DuoStim) in a reduced ovarian reserve population results in a similar euploid blastocyst formation rate: new insight in ovarian reserve exploitation   Filippo Maria Ubaldi, Antonio Capalbo, Alberto Vaiarelli, Danilo Cimadomo, Silvia Colamaria, Carlo Alviggi, Elisabetta Trabucco, Roberta Venturella, Gabor Vajta, Laura Rienzi Fertility and Sterility, Volume 105 Issue 6, June 2016, pages 1488-1495.e1 Objective To compare the euploid blastocyst formation rates obtained after follicular phase (FP) versus luteal phase (LP) stimulation performed in the same menstrual cycle in a preimplantation genetic diagnosis for aneuploidy testing (PGD-A) program in patients

Revised guidelines for good practice in IVF laboratories (2015)

Revised guidelines for good practice in IVF laboratories (2015) The ESHRE Guideline Group on Good Practice in IVF Labs, De los Santos MJ, Apter S, Coticchio G, Debrock S, Lundin K, Plancha CE, Prados F, Rienzi L, Verheyen G, Woodward B, Vermeulen N. Human Reproduction, Volume 31, Issue 4, Pp. 685-686 – Received December 18, 2015. Revision received December 18, 2015. Accepted January 11, 2016.  Epub 2016 Feb 17. ABSTRACT STUDY QUESTION: Which recommendations can be provided by the European Society of Human Reproduction and Embryology Special Interest Group (ESHRE SIG) Embryology to support laboratory specialists in the organization and management of IVF laboratories and the optimization of IVF patient care? SUMMARY ANSWER: Structured in 13 sections, the guideline development group formulated recommendations for good practice in

New approaches for multifactor preimplantation genetic diagnosis of monogenic diseases and aneuploidies from a single biopsy

New approaches for multifactor preimplantation genetic diagnosis of monogenic diseases and aneuploidies from a single biopsy Capalbo A., Rienzi L., Ubaldi F.M. GENERA, Centers for Reproductive Medicine, Rome, Italy GENETYX, Molecular Genetics Laboratory, Marostica, Italy   Fertility and Sterility 2016 Feb. – Volume 105, Issue 2, Pages 297–298 Abstract Preimplantation genetic diagnosis (PGD) is an alternative to spontaneous conception and chorionic villus sampling or amniocentesis for couples at risk of transmitting a defined genetic disorder. It involves the diagnosis of genetic disease on embryo biopsies during an IVF cycle before a clinical pregnancy has been established and represents a preferred reproductive option

The Impact of Biopsy on Human Embryo Developmental Potential during Preimplantation Genetic Diagnosis

33The Impact of Biopsy on Human Embryo Developmental Potential during Preimplantation Genetic Diagnosis Danilo Cimadomo, Antonio Capalbo, Filippo Maria Ubaldi, Catello Scarica, Antonio Palagiano, Rita Canipari, and Laura Rienzi BioMed Research International, 2016 – Published online 2016 January 28 Abstract Preimplantation Genetic Diagnosis and Screening (PGD/PGS) for monogenic diseases and/or numerical/structural chromosomal abnormalities is a tool for embryo testing aimed at identifying nonaffected and/or euploid embryos in a cohort produced during an IVF cycle. A critical aspect of this technology is the potential detrimental effect that the biopsy itself can have upon the embryo. Different embryo biopsy strategies have been proposed.

Early protein profile of human embryonic secretome

1Early protein profile of human embryonic secretome Foresta C, Ubaldi FM, Rienzi L, Franchin C, Pivato M, Romano S, Guidolin D, De Caro R, Ferlin A, De Toni L. Front Biosci (Landmark Ed). 2016 Jan 1;21:620-34 Abstract Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the 3rd day of in vitro culture. 70 proteins were identified in the medium of 48 embryos. Validation by protein array on two pools

Artificial oocyte activation with calcium ionophore does not cause a widespread increase in chromosome segregation errors in the second meiotic division of the oocyte

Artificial oocyte activation with calcium ionophore does not cause a widespread increase in chromosome segregation errors in the second meiotic division of the oocyte  Antonio Capalbo, Christian S. Ottolini, Darren K. Griffin, Filippo Maria Ubaldi, Alan H. Handyside, Laura Rienzi Fertil Steril. 2016 Mar; 105(3):807-814.e2. doi: 10.1016/j.fertnstert.2015.11.017. Epub 2015 Dec 1   ABSTRACT Objective To study the effect of artificial oocyte activation (AOA) on chromosome segregation errors in the meiotic divisions. Design Prospective cohort study with historical control. Setting Private/academic IVF centers. Patient(s) Fifty-six metaphase II oocytes were donated from 12 patients who had undergone IVF between June 2008 and May 2009.