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Largeur intervista alla Dr.ssa Laura Rienzi sulla tecnica della vitrificazione, crioconservazione ovociti
10 Lug
0

Su “Largeur” intervista alla Dr.ssa Laura Rienzi sulla tecnica di vitrificazione degli ovociti

Su “Largeur”  intervista alla Dr.ssa Laura Rienzi sulla tecnica di vitrificazione degli ovociti

Su “Largeur” del 10 luglio l’intervista della Dr.ssa Laura Rienzi sulla tecnica di vitrificazione degli ovociti.

Per leggere l’articolo clicca QUI

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La celiachia non diagnosticata può causare problemi per le donne che provano ad avere un bambino, Genera Roma
10 Lug
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La celiachia non diagnosticata può causare problemi per le donne che provano ad avere un bambino

La celiachia non diagnosticata può causare problemi per le donne che provano ad avere un bambino

Le donne con celiachia non diagnosticata hanno una probabilità maggiore di andare incontro ad aborti spontanei o morte fetale. Lo ha dimostrato uno studio condotto in Danimarca da Louise Grode dell’Horsens Regional Hospital. Tale ricerca, pubblicata sulla prestigiosa rivista Human Reproduction, ha riscontrato che mentre le pazienti che hanno già ricevuto la diagnosi di celiachia rispetto alle donne non celiache hanno le stesse chance di gravidanza e stesse percentuali di rischio di esiti avversi (gravidanze ectopiche, aborti spontanei, etc.), le donne in cui la celiachia non è stata diagnosticata avrebbero una maggiore probabilità di incorrere in un esito negativo della gravidanza. I risultati suggeriscono, dunque, che una mancata diagnosi di celiachia può influire negativamente sulle capacità riproduttive di una donna e che è di cruciale importanza effettuare una diagnosi precoce di tale patologia.

FONTE: HUMAN REPRODUCTION 

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Su “Technologist” “Cryopreservation: a new miracle?” Intervista alla Dr.ssa Laura Rienzi
09 Lug
0

Su “Technologist” “Cryopreservation: a new miracle?” Intervista alla Dr.ssa Laura Rienzi

Su “Technologist” “Cryopreservation: a new miracle?” Intervista alla Dr.ssa Laura Rienzi

 

Su “Technologist” “Cryopreservation: a new miracle?”

Women who want to delay their pregnancies can now freeze their eggs effectively and safely. But success is not guaranteed. Intervista alla Dr.ssa Laura Rienzi

Per leggere l’articolo clicca QUI

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Education program in Reproduction and Development, Monash University, Prato 7-8 luglio 2018, Dr.ssa Laura Rienzi
07 Lug
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Education Program in Reproduction and Development- 30th Anniversary Symposium- Monash University Prato 7-8 luglio 2018. Tra i relatori la Dr.ssa Laura Rienzi

Education Program in Reproduction and Development- 30th Anniversary Symposium- Monash University Prato 7-8 luglio 2018

Si sta svolgendo alla Monash University di Prato,  l'”Education Program in Reproduction and Development- 30th Anniversary Symposium”. Domani interverrà la Dr.ssa Laura Rienzi con una relazione dal titolo “Automation”.

 

PROGRAMMA IN PDF Education program in Reproduction and Development, Monash University, Prato 7-8 luglio 2018

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Eshre 2018 Annual meeting European Society of Human Reproduction and Embryology – ESHRE, Barcellona 1-4 luglio 2018
02 Lug
0

Annual meeting European Society of Human Reproduction and Embryology – ESHRE, Barcellona 1-4 luglio 2018

All'”Annual meeting European Society of Human Reproduction and Embryology – ESHRE“, che si è svolto a Barcellona dall’1 al 4 luglio 2018, ha partecipato numerosa l’equipe dei Centri GENERA. Il Dr. Danilo Cimadomo ha discusso il poster dal titolo “Euploid blastocysts: searching for non-invasive criteria of embryo implantation additional to chromosomal assessment”, mentre la dottoressa Laura Rienzi e Louise Brown, la prima bambina al mondo nata grazie alla fecondazione assistita, sul palco per la premiazione del Grant for Fertility Innovation 2018. Il Dr. Alberto Vaiarelli, invece, ha illustrato i dati clinici dell’utilizzo del protocollo Duostim nei centri GENERA. La relazione dal titolo “No evidences that implantation of vitrified euploid blastocysts is influenced by ovarian stimulation conducted in luteal vs follicular phase: interim analysis of a prospective multicentre study”, ha mostrato come gli embrioni ottenuti dal primo e dal secondo ciclo di stimolazione, hanno le stesse possibilità di risultare in una gravidanza a termine senza complicanze ostetriche e neonatali.

 

PHOTO GALLERY ESHRE 2018

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Preservazione della fertilità: primo passo verso l’ovaio artificiale umano, Congresso ESHERE, GeneraRoma
02 Lug
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Preservazione della fertilità: primo passo verso l’ovaio artificiale umano    

Preservazione della fertilità: primo passo verso l’ovaio artificiale umano

L’ovaio artificiale umano è quasi una realtà: Il risultato è stato ottenuto dal Laboratorio di Biologia riproduttiva del Rigshospitalet di Copenhagen e presentato al congresso della Società Europea di Riproduzione Umana ed Embriologia (ESHERE) che si sta svolgendo in questi giorni a Barcellona. Lo studio, coordinato dalla Dott.ssa Susanne Pors,  ha per la prima volta isolato le strutture che racchiudono gli ovociti immaturi e dopo averle fatte crescere su un’impalcatura di tessuto ovarico privato delle sue cellule, ha dimostrato che sono in grado di funzionare. “Si tratta di di un’importante evidenza preliminare che dimostra la fattibilità di questo approccio per preservare la fertilità delle donne che devono affrontare cure che potrebbero compromettere il loro potenziale riproduttivo” è quanto affermato dalla coordinatrice della ricerca.

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Abstract STUDY QUESTION Are the mean numbers of blastocysts obtained from sibling cohorts of oocytes recruited after follicular phase and luteal phase stimulations (FPS and LPS) in the same ovarian cycle similar? SUMMARY ANSWER The cohorts of oocytes obtained after LPS are larger than their paired-FPS-derived cohorts and show a comparable competence, thus resulting in a larger mean number of blastocysts. WHAT IS KNOWN ALREADY Three theories of follicle recruitment have been postulated to date: (i) the ‘continuous recruitment’ theory, (ii) the ‘single recruitment episode’ theory and (iii) the ‘wave’ theory. Yet, a clear characterization of this crucial biological process for human reproduction is missing. Recent advances implemented in in vitro fertilization (IVF), such as blastocyst culture, aneuploidy testing and vitrification, have encouraged clinicians to maximize the exploitation of the ovarian reserve through tailored stimulation protocols, which is crucial especially for poor prognosis patients aiming to conceive after IVF. LPS has been already successfully adopted to treat poor prognosis or oncological patients through Duostim, LPS-only or random-start ovarian stimulation approaches. Nevertheless, little, and mainly retrospective, evidence has been produced to support the safety of LPS in general. Feasibility of the LPS approach would severely question the classic ‘single recruitment episode’ theory of follicular development. STUDY DESIGN, SIZE, DURATION This case-control study was conducted with paired follicular phase- and luteal phase-derived cohorts of oocytes collected after stimulations in the same ovarian cycle (DuoStim) at two private IVF clinics between October 2015 and December 2017. PARTICIPANTS/MATERIALS, SETTING, METHODS The study included 188 poor prognosis patients undergoing DuoStim with preimplantation genetic testing for aneuploidies (PGT-A). FPS and LPS were performed with the same daily dose of recombinant-gonadotrophins in an antagonist protocol. Blastocyst culture, trophectoderm biopsy, vitrification and frozen-warmed euploid single blastocyst transfers were performed. The primary outcome was the mean number of blastocysts obtained per oocyte retrieval from paired-FPS- and LPS-derived cohorts (required sample size = 165 patients; power = 90%). Mean blastulation and euploidy rates were monitored, along with the number of oocytes, euploid blastocysts and clinical outcomes. MAIN RESULTS AND THE ROLE OF CHANCE Significantly fewer blastocysts were obtained after FPS than LPS (1.2 ± 1.1 vs. 1.6 ± 1.6, P < 0.01), due to fewer oocytes collected (3.6 ± 2.1 vs. 4.3 ± 2.8, P < 0.01) and a similar mean blastocyst rates per retrieval (33.1% ± 30.3% vs. 37.4% ± 30.8%, P = NS). The number of oocytes collected were correlated (R = 0.5, P < 0.01), while the blastocyst rates were uncorrelated among paired-FPS- and LPS-derived cohorts. Overall, a significantly lower chance of producing blastocyst(s) was reported after FPS than after LPS: 67.6% (n = 127/188, 95%CI: 60.3–74.1) vs. 77.1% (n = 145/188, 95%CI: 70.3–82.8; P = 0.05). The mean euploidy rates per retrieval were similar between FPS- and LPS-derived cohorts of oocytes (13.6% ± 22.8% vs. 16.3% ± 23.4%, P = NS). Therefore, on average fewer euploid blastocysts (0.5 ± 0.8 vs. 0.7 ± 1.0, P = 0.02) resulted from FPS. Similar ongoing-pregnancy/delivery rates were reported, to date, after FPS- and LPS-derived euploid single blastocyst transfers: 42.4% (n = 28/66, 95%CI: 30.5–55.2) vs. 53.8% (n = 35/65, 95%CI: 41.1–66.1; P = NS). LIMITATIONS, REASONS FOR CAUTION More studies need to be conducted in the future to confirm the safety of LPS, especially in terms of ovarian and follicular environment, as well as the clinical, peri-natal and post-natal outcomes. Here, we showed preliminary data suggesting a similar ongoing implantation/delivery rate (>22 weeks) between FPS- and LPS-derived euploid blastocysts, that need to be extended in the future, to populations other than poor prognosis patients and using approaches other than DuoStim together with a constant monitoring of the related peri-natal and post-natal outcomes. WIDER IMPLICATIONS OF THE FINDINGS These data, from a paired study design, highlight that LPS-derived oocytes are as competent as FPS-derived oocytes, thereby adding some evidence to support the use of LPS for poor prognosis and oncological patients and to question the ‘single recruitment episode’ theory of follicle recruitment. These findings also encourage additional studies of the basics of folliculogenesis, with direct clinical implications for the management of ovarian stimulation in IVF. TRIAL REGISTRATION None. STUDY FUNDING/COMPETING INTEREST(S) No external funds were used for this study and there are no conflicts of interest. oocyte competence, luteal phase, DuoStim, blastocyst, ovarian stimulation, follicle recruitment, Dr Filippo Maria Ubaldi, Laura Rienzi, Danilo Cimadomo
21 Giu
0

Human Reproduction: Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle

Human Reproduction: Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle

Human Reproduction, dey217, https://doi.org/10.1093/humrep/dey217 , Published: 15 June 2018

Abstract

STUDY QUESTION

Are the mean numbers of blastocysts obtained from sibling cohorts of oocytes recruited after follicular phase and luteal phase stimulations (FPS and LPS) in the same ovarian cycle similar?

SUMMARY ANSWER

The cohorts of oocytes obtained after LPS are larger than their paired-FPS-derived cohorts and show a comparable competence, thus resulting in a larger mean number of blastocysts.

WHAT IS KNOWN ALREADY

Three theories of follicle recruitment have been postulated to date: (i) the ‘continuous recruitment’ theory, (ii) the ‘single recruitment episode’ theory and (iii) the ‘wave’ theory. Yet, a clear characterization of this crucial biological process for human reproduction is missing. Recent advances implemented in in vitro fertilization (IVF), such as blastocyst culture, aneuploidy testing and vitrification, have encouraged clinicians to maximize the exploitation of the ovarian reserve through tailored stimulation protocols, which is crucial especially for poor prognosis patients aiming to conceive after IVF. LPS has been already successfully adopted to treat poor prognosis or oncological patients through Duostim, LPS-only or random-start ovarian stimulation approaches. Nevertheless, little, and mainly retrospective, evidence has been produced to support the safety of LPS in general. Feasibility of the LPS approach would severely question the classic ‘single recruitment episode’ theory of follicular development.

STUDY DESIGN, SIZE, DURATION

This case-control study was conducted with paired follicular phase- and luteal phase-derived cohorts of oocytes collected after stimulations in the same ovarian cycle (DuoStim) at two private IVF clinics between October 2015 and December 2017.

PARTICIPANTS/MATERIALS, SETTING, METHODS

The study included 188 poor prognosis patients undergoing DuoStim with preimplantation genetic testing for aneuploidies (PGT-A). FPS and LPS were performed with the same daily dose of recombinant-gonadotrophins in an antagonist protocol. Blastocyst culture, trophectoderm biopsy, vitrification and frozen-warmed euploid single blastocyst transfers were performed. The primary outcome was the mean number of blastocysts obtained per oocyte retrieval from paired-FPS- and LPS-derived cohorts (required sample size = 165 patients; power = 90%). Mean blastulation and euploidy rates were monitored, along with the number of oocytes, euploid blastocysts and clinical outcomes.

MAIN RESULTS AND THE ROLE OF CHANCE

Significantly fewer blastocysts were obtained after FPS than LPS (1.2 ± 1.1 vs. 1.6 ± 1.6, P < 0.01), due to fewer oocytes collected (3.6 ± 2.1 vs. 4.3 ± 2.8, P < 0.01) and a similar mean blastocyst rates per retrieval (33.1% ± 30.3% vs. 37.4% ± 30.8%, P = NS). The number of oocytes collected were correlated (R = 0.5, P < 0.01), while the blastocyst rates were uncorrelated among paired-FPS- and LPS-derived cohorts. Overall, a significantly lower chance of producing blastocyst(s) was reported after FPS than after LPS: 67.6% (n = 127/188, 95%CI: 60.3–74.1) vs. 77.1% (n= 145/188, 95%CI: 70.3–82.8; P = 0.05). The mean euploidy rates per retrieval were similar between FPS- and LPS-derived cohorts of oocytes (13.6% ± 22.8% vs. 16.3% ± 23.4%, P = NS). Therefore, on average fewer euploid blastocysts (0.5 ± 0.8 vs. 0.7 ± 1.0, P = 0.02) resulted from FPS. Similar ongoing-pregnancy/delivery rates were reported, to date, after FPS- and LPS-derived euploid single blastocyst transfers: 42.4% (n = 28/66, 95%CI: 30.5–55.2) vs. 53.8% (n = 35/65, 95%CI: 41.1–66.1; P = NS).

LIMITATIONS, REASONS FOR CAUTION

More studies need to be conducted in the future to confirm the safety of LPS, especially in terms of ovarian and follicular environment, as well as the clinical, peri-natal and post-natal outcomes. Here, we showed preliminary data suggesting a similar ongoing implantation/delivery rate (>22 weeks) between FPS- and LPS-derived euploid blastocysts, that need to be extended in the future, to populations other than poor prognosis patients and using approaches other than DuoStim together with a constant monitoring of the related peri-natal and post-natal outcomes.

WIDER IMPLICATIONS OF THE FINDINGS

These data, from a paired study design, highlight that LPS-derived oocytes are as competent as FPS-derived oocytes, thereby adding some evidence to support the use of LPS for poor prognosis and oncological patients and to question the ‘single recruitment episode’ theory of follicle recruitment. These findings also encourage additional studies of the basics of folliculogenesis, with direct clinical implications for the management of ovarian stimulation in IVF.

TRIAL REGISTRATION

None.

STUDY FUNDING/COMPETING INTEREST(S)

No external funds were used for this study and there are no conflicts of interest.

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16 Giu
0

XIII Congresso Nazionale SEGI – The Italian job, chirurgia mini-invasiva ginecologica, Avellino 13-16 giugno 2018. Tra i relatori il Dr. Filippo Maria Ubaldi

XIII Congresso Nazionale SEGI – The Italian job, chirurgia mini-invasiva ginecologica, Avellino 13-16 giugno 2018

Al “XIII Congresso Nazionale SEGI – The Italian job”, che si è svolto ad Avellino, tra gli esperti che si sono confrontati sulla chirurgia mini-invasiva ginecologica, anche il Dr. Filippo Maria Ubaldi che è intervenuto con una relazione dal titolo “Preservazione della fertilità nella paziente oncologica: indicazioni e limiti alla stimolazione ovarica per crioconservazione ovocitaria”

PROGRAMMA IN PDF: XIII Congresso Nazionale SEGI – The Italian job, chirurgia mini-invasiva ginecologica, Avellino 13-16 giugno 2018

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L’importanza della Vitamina D in fase pre-concezionale, GeneraRoma
14 Giu
0

L’importanza della Vitamina D in fase pre-concezionale

L’importanza della Vitamina D in fase pre-concezionale

Da uno studio pubblicato questo mese sulla rivista  Lancet Diabetes Endocrinology,  è emerso che i livelli di vitamina D in fase pre-concezionale correlano con la possibilità di ottenere una gravidanza.

Questo risultato era stato già osservato nella popolazione di donne infertili, ma ora viene confermato anche per le donne senza diagnosi di infertilità ma con storia di aborti ricorrenti.

In particolare, lo studio mostra che le donne con livelli sierici di vitamina D ≥75 nmol/L in epoca pre-concezionale hanno maggiori possibilità di ottenere una gravidanza rispetto alle donne con livelli <75 nmol/L. Inoltre, una volta ottenuta la gravidanza, gli autori evidenziano che le donne con maggiori livelli di vitamina D in fase pre-concezionale hanno un minor rischio di aborto.

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Virtual Symposium, The changing Face of ART in the Era of Freeze-All, Dr.ssa Laura Rienzi
14 Giu
0

Virtual Symposium “The changing Face of ART in the Era of Freeze-All”, Madrid 14 giugno 2018 . Special Guest la Dr.ssa Laura Rienzi

Virtual Symposium “The changing Face of ART in the Era of Freeze-All”, Madrid 14 giugno 2018

La  Dr.ssa Laura Rienzi  ha partecipato come “Special Guest” al Virtual Symposium “The changing Face of ART in the Era of Freeze-All” che si èsvolto a Madrid. La dott.ssa Rienzi è intervenuta con una relazione dal titolo “ Freeze-All from lab perspective; are we ready?”

 

PROGRAMMA IN PDF: Virtual Symposium, The changing Face of ART in the Era of Freeze-All

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