Category Archives: Pubblicazioni

Advanced Maternal Age in IVF, Still a Challenge? The Present and the Future of Its Treatment

Advanced Maternal Age in IVF: Still a Challenge? The Present and the Future of Its Treatment

Advanced Maternal Age in IVF: Still a Challenge? The Present and the Future of Its Treatment Filippo Maria Ubaldi, Danilo Cimadomo, Alberto Vaiarelli, Gemma Fabozzi, Roberta Venturella, Roberta Maggiulli, Rossella ...

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A brief history of oocyte cryopreservation: arguments and facts

A brief history of oocyte cryopreservation: arguments and facts

A brief history of oocyte cryopreservation: arguments and facts Benedetta Iussig, Roberta Maggiulli, Gemma Fabozzi, Sara Bertelle, Alberto Vaiarelli, Danilo Cimadomo, Filippo Maria Ubaldi, Laura Rienzi First published: 10 February 2019   https://doi.org/10.1111/aogs.13569 Abstract The term “cryopreservation” refers to ...

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An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploid

An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploidy

An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploid C. Scarica, D. Cimadomo, L. Dovere, A. Giancani, M. Stoppa, A. Capalbo, F. M. Ubaldi, L. Rienzi, R. Canipari Journal of Assisted Reproduction and Genetics, pp 1–13 ...

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How time to healthy singleton delivery could affect decision-making during infertility treatment: a Delphi consensus

How time to healthy singleton delivery could affect decision-making during infertility treatment: a Delphi consensus

How time to healthy singleton delivery could affect decision-making during infertility treatment: a Delphi consensus Ernesto Bosch, Carlo Bulletti, Alan B Copperman, Renato Fanchin, Hakan Yarali, Carlos A Petta, Nikolaos ...

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Diagnostic efficacy of blastocoel fluid and spent media as sources of DNA for preimplantation genetic testing in standard clinical conditions, Equipe Genera,

Diagnostic efficacy of blastocoel fluid and spent media as sources of DNA for preimplantation genetic testing in standard clinical conditions

Diagnostic efficacy of blastocoel fluid and spent media as sources of DNA for preimplantation genetic testing in standard clinical conditions Antonio Capalbo, Ph.D., Valeria Romanelli, Ph.D., Cristina Patassini, M.Sc., Maurizio ...

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Biochemical pregnancy loss after frozen embryo transfer seems independent of embryo developmental stage and chromosomal status, Alberto Vaiarelli, Danilo Cimadomo, Pasquale Patrizio, Roberta Venturella, Giovanna Orlando, Daria Soscia, Adriano Giancani1, Antonio Capalbo, Laura Rienzi, Filippo Maria Ubaldi

Biochemical pregnancy loss after frozen embryo transfer seems independent of embryo developmental stage and chromosomal status

Biochemical pregnancy loss after frozen embryo transfer seems independent of embryo developmental stage and chromosomal status Alberto Vaiarelli, Danilo Cimadomo, Pasquale Patrizio, Roberta Venturella, Giovanna Orlando, Daria Soscia, Adriano Giancani1, ...

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Inconclusive-chromosomal-assessment-after-blastocyst-biopsy-prevalence-causative-factors-and-outcomes-after-re-biopsy-and-re-vitrification

Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience

Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience Danilo Cimadomo, Laura Rienzi, Valeria Romanelli, Erminia Alviggi, Paolo Emanuele Levi-Setti, Elena ...

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Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation

Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation

Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation Danilo Cimadomo, Antonio Capalbo, Paolo Emanuele Levi-Setti, Daria Soscia, Giovanna Orlando, Elena Albani, Valentina Parini, ...

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Impact of Maternal Age on Oocyte and Embryo Competence.

Impact of Maternal Age on Oocyte and Embryo Competence

Impact of Maternal Age on Oocyte and Embryo Competence Danilo Cimadomo, Gemma Fabozzi, Alberto Vaiarelli, Nicolò Ubaldi, Filippo Maria Ubaldi, and Laura Rienzi Front Endocrinol (Lausanne). 2018 Jun 29;9:327. doi: 10.3389/fendo.2018.00327. eCollection 2018   Abstract The overall ...

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Abstract STUDY QUESTION Are the mean numbers of blastocysts obtained from sibling cohorts of oocytes recruited after follicular phase and luteal phase stimulations (FPS and LPS) in the same ovarian cycle similar? SUMMARY ANSWER The cohorts of oocytes obtained after LPS are larger than their paired-FPS-derived cohorts and show a comparable competence, thus resulting in a larger mean number of blastocysts. WHAT IS KNOWN ALREADY Three theories of follicle recruitment have been postulated to date: (i) the ‘continuous recruitment’ theory, (ii) the ‘single recruitment episode’ theory and (iii) the ‘wave’ theory. Yet, a clear characterization of this crucial biological process for human reproduction is missing. Recent advances implemented in in vitro fertilization (IVF), such as blastocyst culture, aneuploidy testing and vitrification, have encouraged clinicians to maximize the exploitation of the ovarian reserve through tailored stimulation protocols, which is crucial especially for poor prognosis patients aiming to conceive after IVF. LPS has been already successfully adopted to treat poor prognosis or oncological patients through Duostim, LPS-only or random-start ovarian stimulation approaches. Nevertheless, little, and mainly retrospective, evidence has been produced to support the safety of LPS in general. Feasibility of the LPS approach would severely question the classic ‘single recruitment episode’ theory of follicular development. STUDY DESIGN, SIZE, DURATION This case-control study was conducted with paired follicular phase- and luteal phase-derived cohorts of oocytes collected after stimulations in the same ovarian cycle (DuoStim) at two private IVF clinics between October 2015 and December 2017. PARTICIPANTS/MATERIALS, SETTING, METHODS The study included 188 poor prognosis patients undergoing DuoStim with preimplantation genetic testing for aneuploidies (PGT-A). FPS and LPS were performed with the same daily dose of recombinant-gonadotrophins in an antagonist protocol. Blastocyst culture, trophectoderm biopsy, vitrification and frozen-warmed euploid single blastocyst transfers were performed. The primary outcome was the mean number of blastocysts obtained per oocyte retrieval from paired-FPS- and LPS-derived cohorts (required sample size = 165 patients; power = 90%). Mean blastulation and euploidy rates were monitored, along with the number of oocytes, euploid blastocysts and clinical outcomes. MAIN RESULTS AND THE ROLE OF CHANCE Significantly fewer blastocysts were obtained after FPS than LPS (1.2 ± 1.1 vs. 1.6 ± 1.6, P < 0.01), due to fewer oocytes collected (3.6 ± 2.1 vs. 4.3 ± 2.8, P < 0.01) and a similar mean blastocyst rates per retrieval (33.1% ± 30.3% vs. 37.4% ± 30.8%, P = NS). The number of oocytes collected were correlated (R = 0.5, P < 0.01), while the blastocyst rates were uncorrelated among paired-FPS- and LPS-derived cohorts. Overall, a significantly lower chance of producing blastocyst(s) was reported after FPS than after LPS: 67.6% (n = 127/188, 95%CI: 60.3–74.1) vs. 77.1% (n = 145/188, 95%CI: 70.3–82.8; P = 0.05). The mean euploidy rates per retrieval were similar between FPS- and LPS-derived cohorts of oocytes (13.6% ± 22.8% vs. 16.3% ± 23.4%, P = NS). Therefore, on average fewer euploid blastocysts (0.5 ± 0.8 vs. 0.7 ± 1.0, P = 0.02) resulted from FPS. Similar ongoing-pregnancy/delivery rates were reported, to date, after FPS- and LPS-derived euploid single blastocyst transfers: 42.4% (n = 28/66, 95%CI: 30.5–55.2) vs. 53.8% (n = 35/65, 95%CI: 41.1–66.1; P = NS). LIMITATIONS, REASONS FOR CAUTION More studies need to be conducted in the future to confirm the safety of LPS, especially in terms of ovarian and follicular environment, as well as the clinical, peri-natal and post-natal outcomes. Here, we showed preliminary data suggesting a similar ongoing implantation/delivery rate (>22 weeks) between FPS- and LPS-derived euploid blastocysts, that need to be extended in the future, to populations other than poor prognosis patients and using approaches other than DuoStim together with a constant monitoring of the related peri-natal and post-natal outcomes. WIDER IMPLICATIONS OF THE FINDINGS These data, from a paired study design, highlight that LPS-derived oocytes are as competent as FPS-derived oocytes, thereby adding some evidence to support the use of LPS for poor prognosis and oncological patients and to question the ‘single recruitment episode’ theory of follicle recruitment. These findings also encourage additional studies of the basics of folliculogenesis, with direct clinical implications for the management of ovarian stimulation in IVF. TRIAL REGISTRATION None. STUDY FUNDING/COMPETING INTEREST(S) No external funds were used for this study and there are no conflicts of interest. oocyte competence, luteal phase, DuoStim, blastocyst, ovarian stimulation, follicle recruitment, Dr Filippo Maria Ubaldi, Laura Rienzi, Danilo Cimadomo

Human Reproduction: Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle

Human Reproduction: Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle Danilo Cimadomo, Alberto ...

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